ATTENTION ⚠️
In Observance of U.S. Holiday, Memorial Day, Agilent Cross Lab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Monday May 30th, 2022. We will resume regular U.S. support hours on Tuesday May 31st, 2022. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.
This service supports the discovery, design and synthesis of ligand and drug molecules using structure-guided approaches. Services include assay design and optimization and screening of compound libraries to discover small molecules that bind to molecular target of interest. Biochemical and structure-based assays are both utilized for lead molecule discovery. Structure-based screening assays include, but are not limited to, Differential Scanning Fluorescence (DSF), Ultra-filtration MS-MS and X-ray crystallographic approaches. For the X-ray based screening approach, screening for crystallization conditions of the target protein is offered as a service along with X-ray structure determination of the target in complex with ligand molecules. To increase the affinity of lead molecules, structure-based design and synthesis of analog compounds is offered along with computational modeling and/or X-ray structure determination of the protein/enzyme in complex with the designed analogs. Purification of the target protein is also offered as well as quaternary structure determination by Multi-light scattering (MALS) or Dynamic Light Scattering (DLS) approaches. Finally, determination of ligand-binding affinity, thermodynamics and kinetics is offered using approaches such as Isothermal Titration Calorimetry (ITC), microscale thermophoresis and surface plasmon resonance.
Andrew Mesecar, PhD
Director
765-494-1924
amesecar@purdue.edu
Hours | Location |
Facility Hours: 24/7 Staffed Hours: 8 am to 5 pm, Mon to Fri |
240 S. Martin Jischke Hockmeyer Hall West Lafayette, IN 47907 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Andrew Mesecar, PhD |
Facility Director
|
765-494-1924
|
amesecar@purdue.edu
|
HOCK 311
|
Nicole Klinkner |
Project Tracking, iLab Invoicing
|
765-496-3595
|
nklinkner@purdue.edu
|
HOCK 267, HOCK 311
|