Life Science Fluorescence Imaging Facility - Lily

Overview of Services

 

The Lilly Hall Zeiss LSM 710 microscope is a state-of-the-art laser scanning confocal microscope well-suited to imaging live cells as well as standard fixed immunofluorescence samples. It is equipped with laser lines at 405, 458, 488, 514, 561 and 633 nm and a 34 channel spectral detector that enables simultaneous imaging of multiple fluorescent proteins. The inverted platform is adapted for a variety of cell culture chambers, and includes a full incubator with CO2 and temperature control. It is outfitted with 40X and 63X apochromat water immersion lenses that are corrected for live cell imaging in culture medium, as well as a 63X planapochromat lens appropriate to fixed material in standard mounting medium. Zeiss Zen software enables advanced imaging including FRET, FRAP, fluorescent protein photoactivation, time lapse and 3-dimensional imaging. The microscope computer is on the university network and transferring image files to your lab computers is simple.

 

 

Leadership

 

Dr. Don Ready
Lilly Hall of Life Sciences, Room 2-231
Purdue University
(765) 494-4975
dready@purdue.edu

 

Location and hours of operation

Hours      Location

Available 24/7 to Trained Users  

     Lilly Hall G403C

Links and Resources

  1.  LSM 710 Confocal Microscope 
  2.  Zeiss Campus 
  3.  Zeiss ZenLE (Zen lite) 

Contacts

Name Role Phone Email Location
Dr. Don Ready
Professor
 
(765) 494-4975
 
dready@purdue.edu
 
LILY 2-231
 
Roger Mahrling
Roger Mahrling
 
(765) 496-0246
 
rmahrling@purdue.edu
 

 

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