Biological Evaluation Shared Resource

Overview of Services 

In cancer biology, mouse models continue to play a significant role in studies of tumor invasion, metastasis and malignant transformation, as well as in studies examining responses to therapy. Key advancements have emerged in the development of animal models for cancer biology, including the advent of orthotopic models for metastasis, transgenic animals that have developmental pathways to tumorigenesis, and state-of-the-art immunocompromised strains. Transplanted human xenografts remain a primary tool for molecular discovery and evaluation. Despite their flaws and shortcomings, xenograft mouse models have played a significant role in cancer drug development in the past three decades, and mouse models will continue to be a foundation in the war against cancer. 

At the Purdue Center for Cancer Research (PCCR), where a vast pipeline of potential new agents for diagnosing and treating cancer has emerged, researchers need a productive and established facility for in vivo testing that can navigate the arena of murine cancer models. 

The mission of the Biological Evaluation Shared Resource (BE-SR) is to provide expert guidance to investigators in grant preparation, model selection and experimental design, and to perform toxicity testing and proof-of-concept efficacy studies for the advancement of their projects using in vivo testing.

If you are new to this core or need to discuss an experiment, please contact Bennett Elzey, Ph.D., Shared Resources Director at belzey@purdue.edu

Services and Pricing

Services Provided:

• Xenograft and Syngeneic models:
• Subcutaneous, intraperitoneal, intracardiac and intravenous Tumor Model Implantation
• Orthotopic Tumor Models:  Bladder, pancreas, prostate, kidney subrenal capsule, intracranial, breast fat pad,
• PDX models
• Efficacy Studies
• Mouse weight
• tumor size monitored by caliper measurements for subcutaneous models and/or in vivo imaging for orthotopic or disseminated models
• In Vivo Hollow Fiber Model
• Toxicity Study, Maximum-Tolerated Dose (MTD) and Pharmacokinetic (PK) Studies
• Drug administration:intravenously (IV), intramuscularly (IM), intraperitonealy (IP), and subcutaneously (SC), Intratracheal instillation, by food and/or drinking water
• In Vivo Imaging studies:
• IVIS Caliper bioluminescence and fluorescence
• Micro CT
• ultrasound
• Euthanasia, necropsy with organ and tissue collection
• Blood draw including cardiac and retro-orbital
• PDX tumor maintenance and expansion
• Cell culture

Pricing:

Acknowledgement

“XXXX” data were acquired by the "XXX" facility supported by P30 CA023168

Getting Started

• Purdue Users: New users: Click LOGIN and use your Purdue ID to register for an iLab account.
• NON Purdue: individuals: Before you register, you must be assigned a Business Partner Number and given a PUID to register/login. Please contact Bennett Elzey, Ph.D. at belzey@purdue.edu.  An email will be sent to you with your PUID and instructions on how to get registered.
• Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

Leadership

Bennett Elzey, PH.D.

Shared Resources Director

Contacts