Transgenic and Genome Editing Facility

Transgenic and Genome Editing Facility (TGEF)

The PCCR’s TGEF Shared Resource is a state-of-the-art facility that offers a large number of services, including the creation of transgenic, gene knock-in and knock-out mouse and rat models for gain-of-function and loss-of-function experiments. Model systems based on transgenic and knock-in/knock-out strategies provide approaches that are developmentally, anatomically and physiologically relevant to human disease, and that can supplement traditional xenograft models for testing new anti-cancer therapies. Genome-edited animal models provide valuable reagents for studies ranging from the regulatory mechanisms governing gene expression patterns to cell-cell interactions, cell cycle control and regulation of signal transduction pathways.

We offer gene editing services in mice and rats by traditional methods such as pronuclear injection of plasmid DNA and gene targeting in ES cells, as well as newer technologies utilizing CRISPR/cas9 for making targeted gene insertions, deletions, modifications and replacements.

We also offer a wide array of supplemental Assisted Reproductive Techniques (ART) to support your mouse and rat research.

If you are new to this core or need to discuss a construct, please contact Judy Hallett, Shared Resources Manager at halletje@purdue.edu

 TGEF Rates 

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 Getting Started

• Purdue Users: New users: Click LOGIN and use your Purdue ID to register for an iLab account.
• NON Purdue Users: Before you register, you must be assigned a Business Partner Number and given a PUID to register/login. Please contact Judy Hallett at halletje@purdue.edu.  An email will be sent to you with your PUID and instructions on how to get registered.
• Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

 Acknowledgement

“XXXX” data were acquired by the "XXX" facility supported by P30 CA023168

 Links and Resources

Contacts