Transgenic and Genome Editing Facility

Transgenic and Genome Editing Facility (TGEF)

The PCCR’s TGEF Shared Resource is a state-of-the-art facility that offers a large number of services, including the creation of transgenic, gene knock-in and knock-out mouse and rat models for gain-of-function and loss-of-function experiments. Model systems based on transgenic and knock-in/knock-out strategies provide approaches that are developmentally, anatomically and physiologically relevant to human disease, and that can supplement traditional xenograft models for testing new anti-cancer therapies. Genome-edited animal models provide valuable reagents for studies ranging from the regulatory mechanisms governing gene expression patterns to cell-cell interactions, cell cycle control and regulation of signal transduction pathways.

We offer gene editing services in mice and rats by traditional methods such as pronuclear injection of plasmid DNA and gene targeting in ES cells, as well as newer technologies utilizing CRISPR/cas9 for making targeted gene insertions, deletions, modifications and replacements.

We also offer a wide array of supplemental Assisted Reproductive Techniques (ART) to support your mouse and rat research.

If you are new to this core or need to discuss a construct, please contact Judy Hallett, Shared Resources Manager at halletje@purdue.edu

 TGEF Rates 

 

MOUSE PCCR Member Rate Internal Rate External Rate

Genome Editing

Transgenic Mouse Production $2,575 $6,230 $9,781
Additional TG Injection Session $715 $1,375 $2,159
CRISPR KI mice $2,760 $7,700 $12,089
CRISPR KO mice $2,095 $5,170 $8,117
CRISPR Validation $60 $260 $408
Additional CRISPR Injection Session $750 $1,725 $2,708
Gene Targeting + Blast Injection $7,500 $18,950 $29,752
Blast Injection of ES Cells $4,900 $11,660 $18,306

 

Assisted Reproduction

Rederivation by Embryo Transfer $235 $410 $644
Embryo Cryopreservation $470 $1,375 $2,159
Speed (Embryo) Cryopreservation $885 $1,740 $2,732
Sperm Cryopreservation $130 $270 $424
In Vitro Fertilization $335 $900 $1,413
Speed Expansion $750 $1,840 $2,889
Cryo Bank per Line charge $18 $22 $34

 

Tissue Culture

Chromosome Counting $100 $360 $565
Mouse Embryonic Fibroblast Production $450 $970 $1,523

 

Husbandry

Production Colony $14 $14 N/A
Mouse Colony Set Up $100 $130 N/A
Animal Shipment (Domestic) + Packaging at cost $140 $140 $220
Animal Shipment (International) + Packaging at cost $235 $235 $369

 

Miscellaneous

Animal Labor Rate $47 $47 $74
Lab Labor Rate $38 $38 $60
Consultation Rate $80 $80 $126


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RAT PCCR Member Rate Internal Rate External Rate

Genome Editing

Rat Transgenic Production $3,810 $8,410 $13,204
Additional TG Injection Session $900 $2,010 $3,156
CRISPR KI Rats $4,000 $9,580 $15,041
CRISPR KO Rats $2,600 $6,040 $9,483
CRISPR Validation $75 $280 $440
Additional  CRISPR Injection Session $1,000 $2,170 $3,407

 

Assisted Reproduction

Rederivation by Embryo Transfer $420 $610 $958
Embryo Cryopreservation $990 $1,900 $2,983
Cryo Bank per Line charge $18 $22 $34

 

Husbandry

Animal Shipment (Domestic) + Packaging at cost $140 $140 $220
Animal Shipment (International) + Packaging at cost $235 $235 $369

 

Miscellaneous

Animal Labor Rate $47 $47 $74
Lab Labor Rate $38 $38 $60
Consultation Rate $80 $80 $126

 Getting Started

  • Purdue Users: New users: Click LOGIN and use your Purdue ID to register for an iLab account.
  • NON Purdue Users: Before you register, you must be assigned a Business Partner Number and given a PUID to register/login. Please contact Judy Hallett at halletje@purdue.edu.  An email will be sent to you with your PUID and instructions on how to get registered.
  • Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

 Acknowledgement

“XXXX” data were acquired by the "XXX" facility supported by P30 CA023168

 Links and Resources

Contacts

Name Role Phone Email Location
Judy Hallett
Lab Manager
 
7654963352
 
halletje@purdue.edu
 
BIND B094
 
Doug Cuttell
Purdue Center for Cancer Research Managing Director
 
7654945022
 
dcuttell@purdue.edu
 
HANS 141
 

Service List


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