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Transgenic and Genome Editing Facility

Transgenic and Genome Editing Facility (TGEF)

The PCCR’s TGEF Shared Resource is a state-of-the-art facility that offers a large number of services, including the creation of transgenic, gene knock-in and knock-out mouse and rat models for gain-of-function and loss-of-function experiments. Model systems based on transgenic and knock-in/knock-out strategies provide approaches that are developmentally, anatomically and physiologically relevant to human disease, and that can supplement traditional xenograft models for testing new anti-cancer therapies. Genome-edited animal models provide valuable reagents for studies ranging from the regulatory mechanisms governing gene expression patterns to cell-cell interactions, cell cycle control and regulation of signal transduction pathways.

We offer gene editing services in mice and rats by traditional methods such as pronuclear injection of plasmid DNA and gene targeting in ES cells, as well as newer technologies utilizing CRISPR/cas9 for making targeted gene insertions, deletions, modifications and replacements.

We also offer a wide array of supplemental Assisted Reproductive Techniques (ART) to support your mouse and rat research.

If you are new to this core or need to discuss a construct, please contact Judy Hallett, Shared Resources Manager at halletje@purdue.edu

 TGEF Rates 

 

MOUSE PCCR Member Rate Internal Rate External Rate

Genome Editing

Transgenic Mouse Production $2,575 $6,230 $9,657
Additional TG Injection Session $715 $1,375 $2,131
CRISPR KI mice $2,760 $7,700 $11,935
CRISPR KO mice $2,095 $5,170 $8,014
CRISPR Validation $60 $260 $403
Additional CRISPR Injection Session $750 $1,725 $2,674
Gene Targeting + Blast Injection $7,500 $18,950 $29,373
Blast Injection of ES Cells $4,900 $11,660 $18,073

 

Assisted Reproduction

Rederivation by Embryo Transfer $235 $410 $636
Embryo Cryopreservation $470 $1,375 $2,131
Speed (Embryo) Cryopreservation $885 $1,740 $2,697
Sperm Cryopreservation $130 $270 $419
In Vitro Fertilization $335 $900 $1,395
Speed Expansion $750 $1,840 $2,852
Cryo Bank per Line charge $18 $22 $34

 

Tissue Culture

Chromosome Counting $100 $360 $558
Mouse Embryonic Fibroblast Production $450 $970 $1,504

 

Husbandry

Production Colony $14 $14 N/A
Mouse Colony Set Up $100 $130 N/A
Animal Shipment (Domestic) + Packaging at cost $140 $140 $217
Animal Shipment (International) + Packaging at cost $235 $235 $364

 

Miscellaneous

Animal Labor Rate $47 $47 $73
Lab Labor Rate $38 $38 $59
Consultation Rate $80 $80 $124


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RAT PCCR Member Rate Internal Rate External Rate

Genome Editing

Rat Transgenic Production $3,810 $8,410 $13,036
Additional TG Injection Session $900 $2,010 $3,116
CRISPR KI Rats $4,000 $9,580 $14,849
CRISPR KO Rats $2,600 $6,040 $9,362
CRISPR Validation $75 $280 $434
Additional  CRISPR Injection Session $1,000 $2,170 $3,364

 

Assisted Reproduction

Rederivation by Embryo Transfer $420 $610 $946
Embryo Cryopreservation $990 $1,900 $2,945
Cryo Bank per Line charge $18 $22 $34

 

Husbandry

Animal Shipment (Domestic) + Packaging at cost $140 $140 $217
Animal Shipment (International) + Packaging at cost $235 $235 $364

 

Miscellaneous

Animal Labor Rate $47 $47 $73
Lab Labor Rate $38 $38 $59
Consultation Rate $80 $80 $124

 Getting Started

  • Purdue Users: New users: Click LOGIN and use your Purdue ID to register for an iLab account.
  • NON Purdue Users: Before you register, you must be assigned a Business Partner Number and given a PUID to register/login. Please contact Judy Hallett at halletje@purdue.edu.  An email will be sent to you with your PUID and instructions on how to get registered.
  • Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

 Acknowledgement

“XXXX” data were acquired by the "XXX" facility supported by P30 CA023168

 Links and Resources


Service List


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